DNA Amplification Fingerprinting: Another Diagnostic Tool?

DNA amplification fingerprinting using two long primers.

DNA-based techniques have great potential for detecting genetic variability between genotypes. However, each approach generates different molecular markers, so the kind and amount of polymorphism detected and the cost and time required vary among them. In general, a good method for DNA fingerprinting to detect genetic polymorphism must generate a complex pattern of bands per experiment, the res...

DNA fingerprinting by infrequent-restriction-site amplification.

Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce strain-specific electrophoretic patterns from c...

Metabolic fingerprinting as a diagnostic tool.

Within the framework of systems biology, functional analyses at all 'omic levels have seen an intense level of activity during the first decade of the twenty-first century. These include genomics, transcriptomics, proteomics, metabolomics and lipidomics. It could be said that metabolomics offers some unique advantages over the other 'omics disciplines and one of the core approaches of metabolom...

Another new tool for the diagnostic bronchoscopist.

Solitary pulmonary nodules (SPNs), defined as rounded lesions <3 cm completely surrounded by an aerated lung, are increasingly identified by modern chest radiography and thoracic CT. As CT screening studies report and the debate over the place of national lung cancer screening programmes intensifies, the need for algorithms to efficiently investigate SPNs will become even more important. Periph...

DNA Fingerprinting by PCR Amplification of HLA Genes